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Exponential-phase cells grown in SCD medium were inspected and imaged with transmitted light and fluorescence using a Leica confocal microscope.
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The cells were grown undisturbed for 14 days, after which the plates were inspected by microscopy and the culture medium was changed every 4 days until the first 10% of wells in each plate became confluent.
These points were inspected to ensure that they represented areas of high, medium and low concentrations of ethnic minority residents.
The wells were inspected regularly during the next 3 weeks and thereafter the medium added was without penicillin and streptomycin.
The stored oropharyngeal swabs were plated onto selective medium, and after 24 48 h of incubation at 37°C (±2°) in 5% CO2, the samples were inspected.
Finally, all plots were inspected visually.
Plates were inspected weekly.
Vaginal plugs were inspected daily.
Cages were inspected daily.
Mice were inspected daily.
The residuals were inspected visually.
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