When 0.5 g DCW (dry cell weight) L−1 initial cells, which was the same inoculation used in the inhibitor-free medium, were inoculated in YP supplemented with the SECS hydrolysate, XH7 consumed 23.16 g L−1 xylose (Fig. 2a; Table 3, Line 5).
Agar plates containing a Mueller Hinton medium were inoculated with Staphylococcus aureus (lux) bacteria.
Flasks containing basal fermentation medium were inoculated from the tube cultures.
Leaves of mustard cabbage sprayed with liquid medium were inoculated with conidia of A. brassicicola and used as a control.
Plates of the medium were inoculated and incubated in aerobic environment for 24 h at 37°C.
Five hundred 500-mL Erlenmeyer flasks each containing 350 mL of culture medium were inoculated with B. vibrans strains.
Five hundred 500-mL Erlenmeyer flasks each containing 350 mL of above-mentioned culture medium were inoculated with P. sulphurascens strains, respectively.
Tubes containing 2.5 ml basal fermentation medium were inoculated from fresh slants of the parent culture F. fujikuroi NCIM 1019 and the selected mutants, generated during mutagenesis.
Baffled 250 mL shake flasks containing 50 mL minimal medium were inoculated with a Y. lipolytica colony from an agar plate.
Eighteen Erlenmeyer flasks each containing 22.5 ml modified fermentation medium were inoculated with 2.5 ml, 48 h old liquid cultures of mutant Mut-4.
Five hundred 500-mL Erlenmeyer flasks each containing 350 mL of above-mentioned culture medium were inoculated with B. vibrans strains, respectively.
