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TAR transformants, grown selective medium, were initially screened by colony PCR using primers for the DBL1alpha domain (Table 1).
The seeding effects seen with both fetuin-A and albumin, manifested only in the presence of exogenously added calcium and phosphate, but not with metastable medium, were initially puzzling.
Saturated liquid cultures grown in YPD medium were initially diluted until OD660 0.05, and serial dilution were prepared as specified in each case.
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He continued: "Now, actually the choice to favor the Internet over broadcast as common medium was initially made in a first draft form in 1994 to 1997 by some of the people who now are running the F.C.C".
To achieve a high 13C enrichment in the biomass obtained, the culture medium is initially stripped of carbon, and labeled 13CO2 is automatically injected on-demand during operation for pH control and carbon supply.
The medium was initially developed for the maximum cell growth based on Luria Bertani (LB) broth and was composed of: glucose 8 g/L, beef extract 7.2 g/L, NaCl 10 g/L, pH 7.0.
For effective production of GABA, the cultivated medium was initially maintained at neutral pH by adding urea during 10 21.5 h of fermentation, and then the pH was let to decline and change spontaneously thereafter.
In the fed-batch mode, 1 L of P2 medium was initially fed into the reactor.
Culture medium was initially replaced 48 hours later and then changed every 2 days.
Briefly, TSB-agar medium was initially poured into petri dishes and left to solidify.
In the clonogenicity study, 10% FCS serum in DMEM medium was initially used to optimise cell culture condition.
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