Sentence examples for medium were infected from inspiring English sources

Cells grown in labeling medium were infected with either Ad-Tet + Ad-MARCH-VIII (heavy-labeled cells) or Ad-Tet (light-labeled cells) alone at an MOI of 200.

Briefly, murine peritoneal macrophages (1×106/ml of complete RPMI-1640 PR− medium) were infected with L. donovani promastigotes as described and then treated with Berberine chloride (0 10 µM) for 48 h; supernatants were used to measure NO using the Griess reagent, NED (0.1% in distilled water) and Sulphanilamide (1% in 5% H3PO4); a standard curve was generated using NaNO2, 0 100 µM [17].

One million Jurkat cells in 1 ml culture medium were infected with HIV-1 corresponding to a 10,000 cpm RT activity.

Cells cultured in antibiotic-free medium were infected at an MOI of 100, centrifuged (2,000  g for 15 min at 37°C), and incubated at 37°C/5% CO2 for 30 min.

For amplifying viral stocks, 1.4 × 106 of Sf21 cells suspended in Sf-900 II SFM (Gibco Invitrogen, Grand Island, NJ) medium were infected at Multipicity of Infection (MOI) 0.1 for 72 h.

For infection of cells, d2 precursor cultures were pre-treated with 10 μM Y-27632 dihydrochloride for 1 h, and thereafter dissociated into single cells by TrypLE. 1 × 10 cells in 1 ml of precursor induction medium were infected with 60 μl of concentrated virus in 15-ml tubes and incubated at 37°C and 5% CO2 for 5 h with occasional mixing.

HiFive cells maintained in Grace medium (Invitrogen) were infected with the different recombinant baculoviruses for the production of the different VLPs.

After adjusting OD600 to 0.5 with SelenoMet plus nutrient mix medium, cells were infected with the methionine-encoded peptide phage library (1 1.2 cells/virion) at 37 °C and 200 rpm for 30 min. Afterward, a methionine biosynthesis inhibitor cocktail comprised of 100 mg/L Lys/Phe/Thr, and 50 mg/L Iso/Leu/Val was added.

Briefly, 4 × 10 fibroblasts were seeded in gelatin-coated 100 mm dishes in fibroblast medium and were infected twice by OCT4, SOX2, KLF4 and cMYC or OCT4, SOX2, NANOG and LIN28 transgene containing retroviruses during a 48 hours period after seeding fibroblasts.

For protein expression, Trichoplusia ni cells grown in suspension in Ex-Cell405 medium (Sigma Aldrich) were infected with equal amounts of both viruses, and cells were pelleted and resuspended for freezing in lysis buffer (40 mM HEPES pH 7.5, 20 mM imidazole, 50 mM NaCl, 10% glycerol, and 2 mM β-mercaptoethanol) after 72 hr.

Intracellular antibacterial assays were done according to the technique previously reported [ 20] as follows: before the beginning of the experiment, bacteria were thawed at 37°C and adjusted to 4 × 10 bacteria per milliliter with RPMI-1640 medium; the monolayers were infected at a multiplicity of infection of 1 10 (cells:bacilli) and incubated for 6 h at 37ºC in a humid atmosphere with 5% CO2.