Cellular response to changes in the concentration of different chemical species in the extracellular medium is induced by ligand binding to dedicated transmembrane receptors.
Expression of the recombinant protein, which was secreted into the growth medium, was induced by the addition of methanol, controlling the feeding rate to maintain a dissolved oxygen tension above 20%%.
When an intense femtosecond laser pulse was focused on culture medium under a microscope, a local explosion of the culture medium was induced at the laser focal point, and a resultant stress wave, with a magnitude around micro-Newton, propagated at the vicinity of the laser focal point (Hosokawa et al., 2011).
'Longquan1', 'Dawuxing' and 'Zaozhong6', were transferred to embryo differentiation medium, embryos were induced only for cv.
H. arsenicoxydans was grown in CDM medium and cultures were induced when required by addition of 0.66 mM As III) for 15 min, 1 hour, 2, 4, 6 or 8 hours.
The top 10 genes induced in serum-free medium were also induced in normal medium, though many more genes were induced in normal medium (Fig. S2B).
Furthermore, in our sphere induction medium, no sphere cells were induced from SK cells when NSF-1 was not added.
To identify the essential role of CA1 for calcification in breast cancer cells, acetazolamide, a chemical inhibitor of CA1, was added into the culture medium when 4T1 cells were induced to produce calcification with OC.
E. coli harboring pET-32 Ek/LIC- omp25, pET-32 Ek/LIC- omp28 and pET-32 Ek/LIC- omp31 plasmids were grown in LB medium and the cells were induced by Overnight Express™ Autoinduction System (Novagen, USA).
