Sentence examples for medium were immediately from inspiring English sources
Samples of the incubation medium were immediately frozen for insulin analysis.
The cover slips mounted on glass slides with 15 µl Mowiol mounting medium were immediately observed via a Leica AOBS TCS SP5 inverted laser scanning confocal microscope.
After incubation, medium was immediately removed and cells were washed with ultrapure water for five times.
After incubation, the medium was immediately removed and cells were washed with ultrapure water for five times.
The remaining medium was immediately replaced with fresh medium and cytotoxicity was determined by MTT assay (see Cell vitality).
Subsequently, the cells were nucleofected by using the U30 program from the nucleofection device and 500 µl of pre-warmed culture medium was immediately added to the cells.
50 ul of complete culture medium was immediately added to pulsed samples and the total solution was transferred to an Eppendorf tube.
The effect of trehalose inclusion in the freezing medium was immediately evident from the morphological appearance of the dry cells observed with Scanning Electron Microscope (SEM).
After illumination, the medium was immediately replaced with DMEM containing 10% FBS.
After irradiation, the donor cells culture was incubated for 10 min, and, then, the medium was immediately collected and transferred to the receptor cells as described above.
The medium was immediately replaced, and spontaneous cell migration was monitored using a Nikon inverted microscope for 18 24 hours, as indicated.
