Aspergillus niger cultures grown in 250 ml flasks containing 100 ml of potato-dextrose broth (PDB medium) were harvested after three weeks.
The bacterial cells grown to OD600nm = 10 in BHI medium were harvested (5,000 rpm for 30 min at 4°C) and disrupted by Mini-BeadBeater16 (BioSpec, Bartlesville, OK, USA) to prepare the cell free extract.
Methylobacterium oryae CBMB20 bacterial culture grown under high C/N and Low C/N medium were harvested at 72 h and washed twice with saline solution and were subsequently used in all the following experiments.
The living and dead cells in the medium were harvested and washed with PBS and then measured by FCM using AnnexinV-FITC/PI apoptosis detection kit (Bender Medsystems, Vienna, Austria) as previously described [35], and 10,000 events were recorded for each FCM analysis.
The gel, cell lysis and culture medium were harvested at 14 days of culturing for analysis.
24 h after the initial dose of heparanase, cells and their conditioned medium were harvested.
After 24 hours, the viruses in the medium were harvested and then replaced with high-serum media.
For titer determination, neurons and medium were harvested from the somal compartment and titered on PK15 cells.
High-titer viral particle-containing medium were harvested 48 h after transfection and used to infect MOLM-14 cells with 8 µg/mL polybrene (Millipore, Billerica, MA).
To assay the enzymatic activities in Bacillus sp. 2 6, L. pentosus DSM 20314 and L. plantarum DSM 20205, cells grown in GSY medium were harvested during the exponential phase.
Worms grown in CeHR medium were harvested at approximately 26 hrs (L2), 34 hrs (L3) and 50 hrs (L4) post L1 seeding according to the growth rate reported by Szilagyi et al. [34].
