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Standing cultures containing 3 ml of M63 medium were grown for 6 days at 25°C in 12-ml polystyrene culture tubes (17×100 mm, Greiner Bio-one).
E. coli cultures in TB medium were grown at 37°C to an optical density (600 nm) of 1.5, induced with 1 mM isopropyl-β-D-thiogalactoside, and allowed to grow for an additional 6 hrs at 20°C.
As for different temperature conditions, overnight log phase cultures of WP3 cells diluted 1000-fold by the same medium were grown at 4°C or 25°C for 3 days to reach an equivalent optical density.
Plantlets recovered after growth on regeneration medium were grown on MS medium without hormones and without bialaphos.
Seedling cultures in liquid medium were grown in ½ Gamborg medium (Duchefa, Harlem, The Netherlands) with or without 0.5% sucrose and the medium was exchanged after 7 days.
BAC clones of HindIII 96-deep-well blocks containing 1.2 ml/well of 2xYT medium were grown overnight at 37°C with shaking at 300 rpm.
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Preculture in reactivation medium was grown aerobically at 30°C with reciprocal shaking at 150 rpm.
A recent IAB survey of media agencies showed awareness of the medium is growing.
Most troubling, industrywide sales of next-generation Blu-ray discs — promoted as a high-definition technology that will restore growth to the medium — are growing but will miss sales projections for the year by 25percentt or more, according to Warner.
It's still an experiment, but given the excitement among folks like Lin and other Hollywood heavyweights, its chances becoming a mainstream medium are growing by the day.
The medium is growing up, and that will inevitably lead to some pains, like Gamergate and other controversies before it.
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