Sentence examples for medium were frozen from inspiring English sources

The cell pellets and medium were frozen at -80°C.

Supernatants of cell medium were frozen for quantification of leptin by ELISA.

The cells were collected as before and the samples free of residual medium were frozen in liquid nitrogen and stored at -80 °C.

Lysates of GCs cells cultured in serum-free medium were frozen at −20 °C, thawed and washed two times in Ellman buffer and used in a 1/8 dilution for activity measurements.

Here the sculptural medium is frozen rosewater gel, flavored with a bit of orange blossom water and vinegar.

Using these cooling methods, sperm of 10 male sterlets diluted with methanol containing cryoprotective medium was frozen.

Conditioned culture medium was frozen (−70°C) until required.

Removed medium was frozen and stored at −80 °C.

Tumour cells were washed three times with RPMI-1640 medium and were frozen in fetal bovine serum supplemented with 10% DMSO until they were used as a target for the cytotoxicity assays.

All the cell lines could be grown indefinitely in routine medium and stocks were frozen in liquid nitrogen for preservation.

The next day, the eyes were embedded in Optimal Cutting Temperature embedding medium and then were frozen, before being sectioned in a cryostat onto clean glass slides.