Sentence examples for medium were flushed from inspiring English sources

Batches of the culture in 120-mL serum bottles, each containing 40 mL of medium, were flushed with mixture gas of N2/CO2 (80 20, v/v) for 5 min and sealed with butyl rubber septums and aluminum crimp seals.

Batches of the culture in 160-mL pressure-resistant serum bottles, each containing 20 mL of medium, were flushed with N2 for 5 min, sealed with butyl rubber septums and aluminum crimp seals, and autoclaved at 121 °C for 20 min.

Bottles with phosphate-buffered medium were flushed with N2 (100%), while bottles with bicarbonate-buffered medium were equilibrated with a mixture of N2 CO2 (80 20%).

For the growth experiments, serum bottles containing the culture medium were flushed with N2 CO2 (80/20) to remove any trace of oxygen in the bottles, capped with thick butyl-rubber stoppers, and autoclaved.

The medium was flushed with CO2 to maintain anaerobiosis.

Phosphate concentration of the medium was kept below 0.5 mM, in order to avoid its possible inhibitory effect on the process, and medium was flushed with argon gas to achieve anaerobic conditions.

Subsequently the medium was flushed with a mixture of nitrogen and CO2 (80 : 20 v/v) for 5 minutes and inoculated with 4 mL inoculum (10% v/v inoculum), before being incubated at 37°C with continuous stirring (150 rpm).

Plugged females were euthanized 3 days after mating and uterine horns were flushed with M2 medium to retrieve blastocysts as well as late morula stage embryos.

Both left and right oviducts were flushed with M2 medium (Millipore, MR-015P-5D) to recover the embryos at embryonic day 2.5 (E2.5).

For live imaging, embryos were flushed using M2 medium (Sigma-Aldrich) at the 2.5-dpc stage.

Embryos were flushed using M2 medium and the zona pellucida was removed if necessary using acid tyrodes solution.