Sentence examples for medium were fixed from inspiring English sources

We assumed that the parameters for the 1D host medium were fixed and known.

Cells that had previously been washed off the medium were fixed with 4% paraformaldehyde solution for 15 min. In order to permeabilize the cells, 0.1% Triton X-100 (Sigma) detergent was added to the prefixed cells for 15 min. Then, the cells were rinsed twice with phosphate-buffered saline (PBS).

Cell monolayers cultured in adipogenic medium were fixed in 10% NBFS for 10 mins.

Jurkat cells in culture medium were fixed 1∶1 (v/v) with 4% paraformaldehyde+0.4% glutaraldehyde pH 7.4 in 0.2 M PHEM buffer (60 mM PIPES, 25 mM HEPES, 2 mM MgCl2, 10 mM EGTA) for 30 min at room temperature.

Fifty one of these SLESs in the minimal and two in the YP medium were fixed by using the global modifications found for GNGs.

Cells treated with osteocyte or adipocyte differentiation medium were fixed in the same manner on days 3, 7 and 14 of treatment.

Final electron donor concentration in the NBAF medium was fixed to 25 mM for both ethanol and pyruvate.

At a word, 3T3-L1 cells were washed twice with phosphate-buffered saline (PBS) after removed the culture medium and were fixed in 10% formaldehyde at room temperature for 10 min, the 10% formaldehyde was discarded and new 10% formaldehyde was added for 1 h.

After removing medium, cells were fixed in 4% buffered paraformaldehyde (pH 7.4) for 30 min. Slides were rinsed twice in PBS, pH 7.4.

After removal of the medium, cells were fixed with 4% paraformaldehyde (PF) for 10 min at room temperature (RT).

After removing labeling medium, cells were fixed and incubated with anti-BrdU-POD working solution for 90 min.