Sentence examples for medium were first from inspiring English sources

Cells growing in serum-containing medium or in serum-free ITS medium were first treated with RA for 48 h.

18°C expression cultures in YG medium were first grown at 37°C, induced with 0,5 – 1mM IPTG and shifted to 18°C for over night expression.

Moreover, five replicate 25 cm culture flasks with standard growth medium were first exposed to IR (1 Gy) and afterwards exposed to standard growth medium supplemented with rhs TRAIL (10 ng ml−1).

Proteins in the cell medium were first processed on a Nickel column to isolate and concentrate the recombinant Fam20a protein and both protein samples were analyzed by immunoblotting using an antiserum specific for the Myc epitope.

Moreover, two replicate 24-well plates with standard growth medium were first exposed to IR (1 or 5 Gy) and afterwards exposed to standard growth medium supplemented with 10 ng ml−1 rhs TRAIL.

To isolate EtBr-resistant mutations, cells grown in YES liquid medium were first inoculated to 40 μl of YES + 2% potassium acetate + 12.5 mg/l of ethidium bromide (YES + EtBr) medium [ 12] in 384-well plates for 2 days before transfer to YES + EtBr Omni plates.

Since the 1890s, when the medium was first introduced, film has been an important means of cultural expression for South African artists.

More than 6 billion CDs have been sold since late 1982, when the new medium was first put on the market by its co-inventors, Philips and Sony, accompanied by the brazen slogan "Perfect sound, forever".

Dubbed XQD, the medium was first announced by Sandisk, Sony and Nikon in November 2010.

A single ring model in an infinite medium is first introduced.

The MH medium was first used in the assays, according to the CLSI recommendations.