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The contents of cellulose, hemicelluloses and lignin in ramie stalk medium were estimated and the changes occurring during fructification were calculated.
ANGPTL2 concentrations in the medium were estimated using an ANGPTL2 Assay Kit (IBL, Fujioka, Japan) according to the manufacturer's instructions.
NO levels in the culture medium were estimated by measuring the concentration of nitrite, its stable metabolite, with Griess reagent as described [ 29].
Cells were then cultivated for another 24 hours and mRNA levels in cell lysates (Table 1) or concentration of cytokine polypeptides released into the medium were estimated (Fig. 5E).
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Biomass in original medium and glutamic acid-containing medium was estimated by determining the dry weight of the mycelia.
For immobilized cells, cell growth in aqueous medium was estimated as the number of cells released from the beads.
In a poly styrene) solvent, the polarity of the medium is estimated to affect little this binding energy.
The SCFA profile of the incubation medium was estimated after 72 h, and from these data the amount of ATP produced in vitro was predicted.
The concentration of free Nile Red added to medium was estimated from the total amount of Nile Red added to the oil phase during particle synthesis.
An upper limit of the degree of silicate crystallinity in the interstellar medium is estimated to be about 5% based on a comparison between a model and infrared observations (Li et al., 2007).
For the free cells, cell growth in aqueous medium is estimated as the total number of cells after the cycle less the cells introduced at the beginning of fermentation.
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