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PC3 cells cultured in medium were either transfected with siRNA or untransfected, and treated with selenium or untreated.

Confluent 25 cellculturelture flasks of RTHDF cells containing 3 ml of cell-culture medium were either incubated with sonicated RTHDF fibroblasts, the supernatant from the sonicate or E.coli 0111 B4 LPS (Sigma-Aldrich).

LNCaP and PC3 cells cultured in medium were either transfected with siRNA (QT00065660 SGCD primers; QT00024157 KCNMA1 primers) or remained untransfected, and treated with selenium or untreated in triplicates.

Cells grown in complete culture medium were either left untreated or treated for different time periods (30 min–12 hrs) with 0,5 mM of either hydrogen peroxide (H2O2) or tert-butyl hydroperoxide (TBHP).

Briefly, 5×105 effector cells (in 0.3 ml of medium) were either added to B721−GFP-H-RasG12V cells (in 0.5 ml of medium) in the lower compartment (done in 24-well plates) or placed in the upper chamber separated from targets.

Overnight cultures in RMG medium were either started from single colonies or from glycerol stocks.

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Similar(53)

Bacterial cultivation medium was either Luria-Bertani (LB) medium or minimal salt basal medium (MSB) (Kongpol et al. 2008).

Results are reported of thermodynamic analyses of a biomass gasification unit in which sawdust is the biomass feed and the gasifying medium is either air or steam.

The medium was either selective yeast minimal medium supplemented with 50 g L−1 glucose and 43 mM NaNO3 YMM-glc-NO3 YMM-glc-NO3 YMM-glc-NO3Rose eTanaka1990) or non-setective yealt complex medium with the addition of 30 g L−1 glucose (YPD).

Culture supernatants were collected on days 5 and 10, when medium was either refreshed and supplemented or not with IL-7.

Co-cultures containing 105 or 2×105 cells were established in wells of 24-well plates by mixing myoblastsFLPe with myoblastsGS.Luc in the specified ratios After an incubation period of 48 to 72 hours the growth medium was either substituted by differentiation medium or by fresh growth medium.

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