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The growth rate measurements in unconsumed medium were done similarly as the measurements in filtrates from the consumed medium.
The pair-wise comparisons between growth rate in filtrates from different sampling points and treatments, and growth rate in unconsumed medium were done using univariate ANOVA for each test species and treatment combination separately, growth rate as a response variable and time as a factor.
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This transfer of cells into XF assay medium was done just a few hours before the XF assays were run.
Perhaps the most original work in this medium was done on clear glass by members of the Beilby family of Newcastle upon Tyne during the 1760s and 1770s.
Preparation of medium was done on vertical autoclave (i.e., Robus Technologies).
The limitation of certain compounds in the growth medium was done to identify the importance of these nutrients for growth and TAG accumulation of R. opacus.
An analysis of the effects of a chemical reaction on heat and mass transfer on a magnetohydrodynamic boundary layer flow over a wedge with ohmic heating and viscous dissipation in a porous medium was done by Kandasamy and Palanimani [17].
IL-8 determination from cell culture medium was done with an ELISA kit (RAB0319, Sigma Aldrich).
Subculture to fresh medium was done at 21-day intervals for a period of six months.
Detection of cytokine concentrations in serum and cultured medium was done with the Endogen SearchLight™ array (Pierce Boston Technology, Woburn, MA, USA).
Application of luciferin in half-strength MS medium was done twice (20 h and 1 h before screening) to differentiating cultures on RD2 medium.
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