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The constituents of 50 mL of this medium were dispensed in flasks of 250 mL capacity.
For exposure, cells suspended in complete growth medium were dispensed into conventional electroporation cuvettes with 1-mm (150-µL volume) or 2-mm (400-µL volume) gap between the electrodes (BioSmith Biotech, San Diego, CA).
One hundred microliters of UMMt medium were dispensed into wells 1 to 20 and 50 μl of S.Mh were dispersed into wells 6 and 7.
After evaporation of the ethanol, aliquots (200 μL each) of the rYES assay medium were dispensed into each well, and the plates were sealed, shaken, and incubated in the same manner as the reference chemicals.
Fifty mL of asparagine dextrose salts broth medium were dispensed in 250 mL Erlenmeyer conical flasks, sterilized, inoculated, and incubated at 30°C for 5 days in a rotatory incubating shaker at 150 rpm.
Diluted aliquots (∼4 × 10 cells in 50 μl of the same medium) were dispensed into wells of a 96-well plate containing 50 μl of the same medium or 50 μl of a twofold dilution series of GE in the same medium (final concentrations = 0 and 8 8000 μg/ml), and were incubated 16 hr at 37°C with shaking under the same conditions.
Similar(53)
For this purpose, the production medium was dispensed into flasks of 250 mL capacity; each flask containing 50 mL of liquid medium was adjusted at pH 6.5.
Under aseptic conditions in the laminar flow hood, the medium was dispensed into 150 mm pre-sterilized petri dishes to yield a uniform depth of 4 mm.
The biofilm formation was carried out using the P63 strain of Pseudomonas aeruginosa and the M63 minimal medium [M63 salt 12 g/l KH2PO4, 28 g/l K2HPO4, 8 g/l (NH4) SO4, 1 mM MgSO4, 0.5%] 150 μl of M63 liquid medium was dispensed into two 12-well plates, and the seed culture of the clinical cultures cultured for 24 h was inoculated at 2% and cultured at 30 °C for 72 h.
The medium was dispensed into the culture tubes while being gassed with 100% CO2, at 9.5 ml of medium per tube, and the tubes sealed with blue butyl septum stoppers and aluminium seals (Bellco), with a headspace of 100% CO2.
The procedures for the co-inoculation assay were similar to those of the pre-infection assay above with the following modifications [15]: 70 µl of screen medium was dispensed into each well of a 96-well non-binding half area plate (Corning) using a MultiDrop Combi Plate Filler (Thermo Scientific) equipped with a standard dispensing cassette (Thermo Scientific).
More suggestions(15)
medium were distributed
media were dispensed
medium were studied
medium were preincubated
medium were introduced
medium were sterilized
medium were poured
medium were included
medium were extracted
medium were tested
medium were served
medium were removed
medium were recorded
medium were used
medium were injected
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