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After 24 hour serum starvation, medium were discarded and AoSMCs washed and resuspended with fresh DMEM medium.
Sixty ml of the medium were discarded, and chloroquine was added to the remaining medium at a final concentration of 25 μM.
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The growing medium is discarded coffee grounds from Peet's Coffee that would otherwise go into a landfill.
Mr. Dinges's medium is discarded Apple laptops — specifically, shiny white MacBooks on which he inscribes images, sayings, sometimes even warnings.
Before the assay, culture medium was discarded.
After 72 h, cell culture medium was discarded and the cells were washed twice with PBS.
The medium was discarded and 50 μL 1× lysis buffer was added to each well.
Culture medium was discarded and was replaced with 150 μL DMSO.
The medium was discarded and replaced with DOX solution and DOX nanoparticles.
Following incubation, medium was discarded and cells were washed three times in PBS.
Subsequently, the cell medium was discarded, and the cells were lysed with 0.004 N HCl in isopropanol.
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CEO of Professional Science Editing for Scientists @ prosciediting.com