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After cryopreservation, the post-thaw cells with CPA (betaine or DMSO) solution added four-fold amount of medium were directly infused into the culture flask for cell recovery (Fig. S5).
A midline incision of the anterior abdominal wall was made, and 2 × 106 cells in a total volume of 0.1 mL of a serum-free medium were directly injected into the left lobe of the liver under anesthesia by pentobarbital sodium.
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Direct absorption solar collector (DASC) is a new generation of solar thermal collectors in which the transport medium is directly exposed to the incident solar radiation.
The greater contact of the cells with the culture medium is directly related to cell viability and to the increase of proliferation rate.
The greater contact of the cells with the culture medium is directly related to oxygen diffusion and thereafter with the cell viability and the increase of proliferation rate.
To infect SOX9+ BCs, 0.5 mL lentivirus containing medium was directly added to 2 mL cell culture medium with 10 μg/mL polybrene and incubated for 12 h.
First, the amount of lactate secreted into the culture medium was directly measured in Patient 1 fibroblasts.
For measuring luciferase activity in the medium, 50 µl medium was directly mixed with 50 µl of the prepared luciferase reagent.
Viability of the AEY1017 telomeric reporter strain on FOA medium is directly proportional to silencing activity (Meijsing & Ehrenhofer-Murray, 2001).
Virus-containing medium was directly added to ATDC5 cells that had been plated to 80% confluence in maintenance medium 24 hours prior to infection.
These results show that the amount of IL-12 p40 secreted into the medium is directly proportional to the amount of the mRNA present in the cells.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com