Sentence examples for medium were diluted from inspiring English sources

Overnight E. coli cultures, grown in LB medium, were diluted 100-fold in LB medium and shake-incubated at 37 °C, 180 rpm, to a OD600nm = 0.6 cell density.

Overnight grown yeast cells in YPGal medium were diluted to OD600 = 0.3 and grown for 4 h at 30°C.

Total cell lysates and culture medium were diluted 1∶50, and hPGRN concentrations were detected with an ELISA kit (Alexis Biochemicals) according to the manufacturer's instructions.

The clear supernatant and the medium were diluted with 9x vol. of 0.1% trifluoroacetic acid (TFA) and were passed through activated Sep-Paks by gravity.

For ether treatment, cells in culture medium were diluted 1∶2 with ethyl ether, mixed, and after 10 minutes, samples were removed, diluted, and titered.

Exponentially growing cells in PAB medium were diluted back into fresh PAB medium with or without 1% xylose to inducce overexpression of GFP-MinC/MinD.

After incubation for 24 h, 1 mL medium was diluted, and 0.1 mL each of 107 dilutions was plated on nutrient agar and incubated for 24 30 °C in dark, and colonies were directly counted and expressed as CFU/mL.

Briefly, the medium was diluted by Milli-Q water×200; 25 µl of the diluted medium and 100 µl osteocalcin antiserum were placed in 96 well EIA plates and incubated at 4°C for 18 24 hours.

Clarified cell culture medium was diluted with 1/10 volume 10× PBS pH 7.4.

Clarified cell culture medium was diluted with 1/10 volume Buffer A (500 mM sodium phosphate, 5 M NaCl, pH 8.0).

The collected medium was diluted to 5 ml with PBS and further diluted by 15-fold with ethanol.