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First, a count of n = 50.000 commercially available osteoblasts (PromoCell, Heidelberg, Germany) per millilitre of culture medium were cultured on the bottom of the cell-bearing surface (glass panel).
Secondly, after extensive washings with phosphate buffered saline (PBS), chorionic villi explants (3g/20 ml of culture medium) were cultured for 24 hours at 37°C in humidified atmosphere containing 5% CO2.
pLXSN or pLXSN- Mash1 was transfected into the φ2 packaging cells, and SH-SY5Y cells (1×106 cells) infected with virus-containing culture medium were cultured in the medium containing 500 µg/ml G418 (Sigma Chemical Co., St . Louis MO, USA).
Cells (1 × 10/cells in 2 ml culture medium) were cultured at 37°C and 5% CO2 in a humidified atmosphere.
Cells seeded in 6-well plates at 20,000 cells/ml in 2 ml culture medium were cultured in the absence (Control) or presence of N7 and N6L.
YE-PC8-infected ΔGli36 cells, which were previously incubated in standard tissue culture medium, were cultured in either DMEM containing 10% FBS or DMEM containing 10% FBS and 150 μg ml−1 phosphonoacetic acid (PAA).
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The plates were incubated overnight at 37°C and the next day the opposite compartment with MS solid medium was cultured with 3 4 day old A. thaliana Col-0 plants.
Then a single colony was inoculated in TSB medium and the medium was cultured in a rotary shaker at 37 °C.
C. albicans in Sabouraud medium was cultured overnight in the presence or absence of CSC at various concentrations (0, 10, 30, or 50%).
A cell suspension (1 × 10 per ml) in serum-free medium was cultured on the basement membrane after removing the rehydration media.
Briefly, 5 × 10 cells/well in 100 μL of medium was cultured in 96-well microtiter plates (BD-Falcon Biosciences, Lexington, TN).
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