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At 24 h pi, 5 g/L of the initial free fatty acids in the REG-GB medium were consumed, indicating a simultaneous consumption of glycerol and free fatty acids, while 2.5 g/L of free fatty acids in REG-FFA medium were consumed in the same period (Fig. 2b).
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Experiments in monitoring TV watching have a long academic history as well, and many documentaries, including the influential 1986 series Open the Box, have explored how the medium was consumed.
It is the secret sauce that has made social networks like Facebook, Twitter and Pinterest such phenomenal successes.Inevitably, turning television into a platform for social networking will affect the way the medium is consumed.
At this moment, about 1/3 glucose in the initial medium was consumed.
When the medium was consumed, additional GO-dispersed culture medium was sprayed onto the surface of the newly prepared BC/GO film, and the second layer of the BC/GO film was produced.
After 27 h incubation, about 20% of the total organic C in the initial medium was consumed and the average MW of PAM was reduced from 2 × 106 to 0.5 × 106 by microbial degradation.
An analysis of glucose contents in the medium by HPLC revealed that glucose was consumed at a constant rate until all the glucose in the medium was consumed after 60 or 72 h of cultivation; however, the production of CDM and PHB increased until after 36 h of cultivation and mostly stopped after this period for the control and samples treated with an additional 0.3 M NaCl.
Only 2.3 g l-1 glucose in the medium was consumed, and neither ethanol nor xylitol could be detected.
The totality of glucose contained in the YMD medium was consumed for all the condition tested with a consumption rate inversely proportional to the growth.
RNA was isolated at the midtime of fermentation when half of the sugar present in the medium was consumed (equivalent to 45 g CO2), and then labeled with the Chipshot direct labeling and cleanup kit (Promega).
The cultivations in this study were carried out in batch mode and therefore medium compounds were consumed over time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com