Sentence examples for medium were combined from inspiring English sources

Cells, washes and culture medium were combined.

Cells floating in the medium were combined with the adherent cell layer, which was then trypsinized.

Equal amounts of the total RNA from cultures grown in each medium were combined to create a single pool of total RNA.

Oligomer Oligofectamine™ complexes and aliquots of 2×10 HeLa cells in 2 ml of culture medium were combined and incubated for 5 min at room temperature.

Hippocampal neurons were transfected on DIV3 (for morphometry) or DIV5-8 (for videomicroscopy) as follows: for each coverslip, plasmid DNA (2 μg) and Lipofectamine 2000 (1.25 μl, Life Technologies) in Neurobasal medium were combined and incubated for 30 min. After the addition of complete Neurobasal medium containing B27 supplement, the mix was applied onto the neuronal culture for 3 hr at 37°C.

The wash medium was combined with the supernatant of the previous step.

Fresh medium was combined 1 1 (v/v) with filtered conditioned medium from earlier BEC cultures.

For each reaction, 0.25 µl Lipofectamine 2000 and 25 µl OptiMEM I minimal growth medium (Invitrogen) were combined and incubated at room temperature for 10 minutes.

There were only few participants (five men and nine women) with a low level of education, and therefore the low and medium levels were combined.

Growth medium and cells were combined and centrifuged at 500 × g for 5 min, and the resulting cell pellets were washed with PBS to remove trypsin.

6 For the purposes of the current analysis the high and medium dose arms were combined and compared with the low dose arm.