The bacterial colonies grown on different types of medium were collected and maintained in Nutrient slant agar for further analysis.
The PHB-producing cells grown in the 50 mL medium were collected by centrifugation (4°C at 3,200×g for 10 min) to an optical density at 600 of 4.0.
At a specified culture period, such as the 6th day, mycelia in the culture medium were collected by filtration and rinsed with pH 2 water (50 ml) to scour off the residual nutrients as well as nonionic surfactants.
For the extraction of genomic DNA, cells cultured in PD medium were collected by centrifugation, and 3 volumes of TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) with 2% sodium dodecyl sulfate (SDS) were added to the cell pellet.
For detection of mCherry displayed on the cell wall of Y. lipolytica using YlCWP1, yeast cells in the culture medium were collected and washed three times by centrifugation at 16000 x g for 2 min at 4°C using phosphate-buffered saline (PBS pH 7.4).
The progeny virions released into the culture medium were collected, purified and analyzed by SDS-PAGE.
The cell lysate and culture medium were collected 30 hr post-transfection.
Following incubations, aliquots of medium were collected and subjected to a specific ELISA for IL-6.
Aliquots of medium were collected on 1, 2, 3, and 4 days post-infection.
Bacteria grown in THY laboratory medium were collected in ML, LL and S phase from three independent cultures (biological replicates).
Briefly, 40 µL of transport medium were collected from each of five feather swabs and pooled in a single tube.
