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The particles and filter medium were coated to model poor wetting.
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A graphene/carbon nanotube hybrid material stabilized in an aqueous medium, was coated on carbon fibers by anodic electrophoretic deposition.
Flat wells plates (medium binding) were coated overnight at 4°C with 50 μl polypeptide solution (125 ng/well) in sodium carbonate buffer (50 mM, pH 9.6).
The SGBS cells were seeded into culture medium flasks or plates, which were coated with a solution of 10 µl/ml fibronectin and 0.05% gelatine in phosphate-buffered saline (PBS).
Peptides and fibrinogen protein from human plasma (Calbiochem, Gibbstown, NJ, USA) were coated onto medium-binding 96-well flat-bottom polystyrene plates (Costar, Corning Inc, Corning, NY, USA) at 1 μg peptide/ml at 4°C overnight.
We then transferred the seedlings to microscope slides that were coated with MS medium, and grew them for two additional days.
Before adding medium and mPCKS, 12-well plates were coated with 10% bovine serum albumin (BSA) in milliQ water for 30 min.
Medium binding, 96 well ELISA plates (Greiner) were coated with 50 µl of a 1 µg/ml ICSM18 antibody solution in coating buffer.
Plates were coated with Matrigel (BD Biosciences), and medium was changed daily.
Briefly, the wells were coated with 0.5 ml of 0.6% agarose containing serum-free medium.
Transwell inserts were coated with 40 μL Matrigel diluted 1 3 (v/v) with serum-free medium.
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