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The concentrations of carbon source (glucose), inorganic nitrogen ((NH4 2HPO4), potassium (KH2PO4) and magnesium (MgSO4·7H2O) sources in medium were changed according to the central composite rotatable design consisting of 29 experiments, and the biomass yield was calculated.
hES cell culture medium for hFLSCs feeders was identical to the medium used in MEFs feeders, and the medium were changed daily.
hES cell culture medium for bFGF-hFLSCs feeders was also identical to the medium used in MEFs feeders but removal of the bFGF, and the medium were changed daily by partial medium changes.
The medium were changed twice a week.
Medium were changed 12 24 h after, and experiments were performed 48 h after transfection.
Medium were changed every two days, and colonies of late EPCs (lEPCs) appeared after 2 3 weeks.
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Growth medium was changed every other day.
The medium was changed every day.
The medium was changed every other day.
Half volume of the medium was changed every 2 3 days.
The fresh culture medium was changed three times a week.
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