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Hence, exponentially growing cells washed in Fe-free medium were challenged for 48 h in liquid medium containing 0 to 2 μM of (NH4 FeH2C6H5O7.
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When cells grown in fermentable medium are challenged with nitrogen starvation, bulk autophagy is rapidly activated.
To determine effects of furfural on C. beijerinckii 8052 growth and ABE production at acidogenic and solventogenic phases, a C. beijerinckii 8052 culture grown in P2 medium was challenged with furfural, and changes in cell density, acid production and ABE production were measured relative to cultures grown in P2 medium without furfural.
The following morning, the medium was replaced and cells were challenged as mentioned above.
This medium was replaced and cells were challenged with fresh, serum-free RPMI media containing the following drugs at the specified concentrations: serotonin (3.125, 6.25, 12.5, 25, 50 μM), ritanserin (3.125, 6.25, 12.5, 25, 50 μM), and concurrent serotonin (12.5 μM) and ritanserin (3.125, 6.25, 12.5, 25, 50 μM).
After 2 hours in culture, the medium was removed and the cells were challenged with CHIKV (m.o.i. of 5) in the presence of the drug.
HUVECs and HUVECs with transient TLR2 knockdown were challenged with basal medium (GM2) alone or GM2 cell culture media containing 1 500 nM CEP-DPPE for 12 h in 5%CO2/95%% air at 37 °C.
Twenty-four hours post-transfection cells were challenged with stimulants in fresh medium.
As television has come to be recognized as a legitimately artistic medium, viewers have been challenged to rise to the occasion – to meet those ambitions with a level of savviness and interpretive skill that wasn't required when we could relax into the laugh tracks.
Confirming the presence of enamel paint such as Ripolin in early twentieth century artworks scientifically through binding medium analysis is challenging because, until the end of the Second World War, the most widely used house paints were oil-based and chemically similar to artists' oil paints.
Finding a happy medium will be challenging for healthcare innovators in the developing world, but Shantha has shown that it can be done.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com