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10 (8.08 g/L) where concentrations of crude glycerol, urea and magnesium sulfate in the medium were as follows g/L: 36.8; 2.0 and 0.2.
The time courses of polymer synthesis for each cultivation condition in BM medium were as follows (Figure 3): (1) Anaerobic: cell growth and addition of ammonium hydroxide almost stopped after 48 h cultivation time.
The routine culture of ES cells, formation of EPL cells and the production of MEDII conditioned medium were as described previously [11].
Treatments with specific DNA-damaging agents (while shaking at 28°C in the growth medium) were as follows: Hydrogen peroxide: final concentration of 2 mM, the treatment time was fixed for 20 minutes.
Ingredients used to prepare the serum-free medium were as previously described.
The final allergen cocktail concentrations in culture medium were as follows: Der p1 and Der f2 (dust mite), 1 μg/mL; Fel d1 (cat), 1.25 μg/mL; ragweed, 125 μg/mL.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com