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Five differing volume system ratios of the culture cells' extracellular medium were applied for 24 and 48 h of incubation in this study.
Rat hippocampal neurons, cultured at low density in serum-free medium, were applied to the growth matrix of PA22-2-coated substrates and, within 1 3 h of culture, formed a network-like pattern that more or less matched the printed grid.
In 3 of the 5 vessels, B31-A and B31-A3 spent medium were applied to the same vessel sequentially.
Fifty μl of culture medium were applied to determine the background impedance (30 min).
However, in all these cases saccharification of pure cellulose (e.g. Sigmacell-50) or washed fibers, in defined fermentation medium, were applied.
5 × 104 [H] Thymidine-labeled cells in 100 μL RPMI 1640 medium were applied in the upper compartments, in triplicate wells.
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No contrast medium was applied during MRI.
Then, the extraction medium is applied into the test system.
The principle of effective stress for porous medium is applied to analyzes the fluid-structure coupling in grouting.
The thermal-controlled nanostructured reaction medium is applied to the ene reaction, [4+2] cycloaddition and sulfide oxidation.
The cells were left for 1 h at 37 °C to allow attachment after which 1 mL of pre-warmed culture medium was applied on each well.
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