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Exact(4)
Seedlings in vanadate-treated medium were allowed to grow for approximately 2 weeks.
Cells at a density of 15 000 cells/150 μL/well in a 96-well plate in RPMI growth medium were allowed to attach for 24 h.
These flasks of medium were allowed to equilibrate at 37 °C in a humidified 95%air/5%% CO2 atmosphere for at least four days before the pH was determined.
Endothelial cells (HMEC-1 or HUVEC) plated on the upper chamber containing serum-free medium were allowed to migrate to the lower chamber containing MDA-MB-231 breast cancer cell-derived conditioned medium (231-CM, serves as a chemo-attractant) plus various concentrations of THL.
Similar(56)
Abrasive medium is allowed to fall freely on monolithic glass and induce a random surface flaw population.
Dividing time into activity windows is only used as a reference to specify, with relatively low accuracy, when attempts to access the medium are allowed to be made.
The medium was allowed to solidify and then the petri plates were spread with Bacillus subtilis and Escherichia coli separately in a laminar air flow hood.
The importance of bandwidth management is so high that in the new DVB-RCS2 standard, even random access to the medium is allowed in order to optimize resource usage.
After sterilization, the medium was allowed to cool at room temperature and inoculated with 7% suspension of S. diastatochromogenes and incubated at 30 °C with shaking speed of 165 rpm for 96 h.
The staining medium containing 5 µM LysoSensor probe in 5 mL growth medium was allowed to equilibrate to 37°C in a water bath for 30 minutes.
The ammonia in the medium was allowed to decrease to zero though nitrogen flow into the bioreactor was still positive due to base addition.
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