Sentence examples for medium we removed from inspiring English sources

To investigate the role of individual components in 5i/L/FA medium, we removed single kinase inhibitors or growth factors from a clonal line of OCT4-ΔPE-GFP+ cells derived in the absence of transgenes.

We applied 10 µM of bromodeoxyuridine into the cell culture medium when we removed the PDMS sheet or 24 hours after the removal.

Since epidermal growth factor (EGF) and fibroblast growth factor (FGF) are established critical components of stem cell culture medium, we next removed these growth factors to determine effects on GSC propagation.

After conversion of MTT, we removed the medium and added 200 μL DMSO to the insoluble fraction.

After four hours of incubation at 37°C, we removed the medium from both flasks and washed the cells twice with phosphate-buffered saline (PBS).

After 1, 4, 7 and 14 days' incubation (n = 3 4), we removed the medium, added 30 μl/well MTT solution in darkness, then incubated for 3 h at 37°C.

After serum deprivation in DMEM containing 5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL sodium selenite, and 0.1% BSA for 48 hr, we removed this medium and added new DMEM/0.1% BSA with or without the appropriate reagent or vehicle control (ethanol).

For the computation of flux distributions under varying media conditions, we started from a rich medium, removing medium components one by one under the condition that biomass production is not disrupted until a minimal medium composition was reached.

The same volume of the appropriate medium was added to replace the medium removed.

After treatments, culture medium was removed and washed with PBS.

Then the medium was removed and the substrates were rinsed with PBS three times.