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To further investigate gene targets of overexpression for enhanced TAG production in MG medium, we performed APGC algorithm with the use of newly constructed model iYL_2.0.
To test the components of the condition medium, we performed mass spectrometry and then obtained a list of candidate proteins (Fig. 2E).
In order to stablish the best culture medium, we performed a kinetic study aiming to determine the time to achieve the maximum value of the enzyme activity and the growth curve for this microorganism.
To investigate whether rhamnolipid yield in ZS1 isolate was altered by change of glucose supplement concentration in MS medium, we performed analyses of cell biomass and biosurfactant activity by ZS1 in MS medium supplemented with various concentrations of glucose (see "Materials and methods").
To address the question if there is a transcriptional induction of any of the catalase genes in the XVM2 medium we performed semiquantitative RT-PCR analysis with early exponential phase cultures.
Because no clear effects of αSyn or the other brain proteins could be observed in WT BY4742 cells grown in normal inducing medium, we performed growth experiments in the presence of DMSO.
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In order to investigate the extent to which the physiology (e.g., metabolic activity) of primary cancer cells was influenced by such an unstable culture condition (e.g., culture medium pH), we performed the following experiments.
In order to demonstrate that SCOS is capable of retrieving the dynamic properties of a medium, we have performed measurements in tissue simulating phantoms in a similar fashion as previous DCS experiments [ 16, 17].
To determine the effect of pUC/pU2 bicistronic constructs on angiogenesis of conditioned medium from meningioma cells, we performed vessel formation assay in human microvascular endothelial cells (Fig. 1D).
In order to examine RIBE in hSC grown in media collected from directly IR-exposed hSC, we performed medium transfer studies as described in the Materials and Methods section.
We performed medium-depth (8×) whole exome sequencing in 1,000 cases with type 2 diabetes, BMI >27.5 kg/m and hypertension and in 1,000 controls (stage 1).
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