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In our recent series of solar carbide synthesis for Si and d-group transition metals (Ti, Zr; Cr, Mo, W) using graphite (G) or amorphous carbon (aC) as carburising medium, we observed some evidences indicative of involvement of photochemical effects in the reaction process.
In ectoderm explants grown in control medium we observed the appearance of numerous hair follicles (arrows in Fig. 5A).
Particularly in a defined medium, we observed only a partial decrease in biofilm formation in the ΔchpBIK, ΔrelBE, and ΔyefM-yoeB mutants (Figure S4).
However, in TWEAK antibody supplemented culture medium, we observed a significant increase of both NKp46 (Figure 6A) and TGF-β1expression (Figure 6B).
After culture in Huh-7 cells conditioned medium we observed an up-regulation of αSMA in aMSC and pMSC already at 10 d (Fig. 4B).
After 16 hours shift to glucose-containing medium, we observed accumulation of 35S and 23S pre-rRNAs whereas 20S pre-rRNAs were significantly reduced (compare lanes 1 and 3, Fig. 4A) confirming that Nop14p is involved in an early step of pre-40S maturation.
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Addition of ATP to a calcium-free medium does not increase the [Ca2+]c levels, however in a calcium medium we observe an increase in cytosolic calcium.
Finally, when we examined the potential of BSG to replace the constituents of a medium formula, we observed that simultaneously adding BSG, omitting dextrin, and reducing the other ingredients concentration in the culture medium improved the production of α-amylase and made the production process more economical.
After exposure with high but not with medium NoA we observed a statistically significant decrease in maximum mitochondrial respiratory capacity (E-state, C 133 (118; 148) vs. high NoA 111 (106; 113), and medium NoA 129 (123; 140), P < 0.05 C vs. high NoA).
Interestingly, when we incubated MycΔ/Δ cells in medium alone, we observed 3- to 5-fold increase in antibody secretion relative to Mycfl/Δ or wild-type B cells, which indicated that the Myc deletion facilitated terminal differentiation to antibody-secreting plasma cells in vivo (Fig. 3E).
In plating tests on agar medium, we rarely observed in the harvested spore solutions contaminations by other fungi (mostly none to up to in 10% of samples in different test series) and infrequently by bacteria (in between 0 and 22% of samples of test series) which could mostly be suppressed by bactericides.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com