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In addition to using RNA from mycelia grown in rich medium, we included RNA from mycelia following heat and cold shock.
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To test whether cells migrate in an FCS-directed manner, we included FCS-free medium controls for each of the tested cell lines, without noting cell migration in any case (data not shown).
Due to the limitations of the print medium we couldn't include all the banners we wanted to without either making them teensy-tiny or turning Vice into an Ed Hall fanzine, but here on the internet we can do whatever the fuck we want.
Wells filled with growth medium were included as negative controls.
As a negative control cell-free medium was included.
Growth control wells containing medium were included in each plate.
An additional non-infected medium was included for sterility check.
The EmtR1 cell medium also included 1 μM of emetine.
The culture medium included Dulbecco's modified Eagle's medium (DMEM, Gibco, USA) with 15% fetal bovine serum (Gibco, USA).
Twenty-four hours after plating, we changed the medium to include the test agents.
We introduced CPF or CPF photolysis products for 1 hr in undifferentiated cells, and then, to initiate the measurement of DNA synthesis, we changed the medium to include 1 μCi/mL [H]thymidine (specific activity, 2 Ci/mmol; GE Healthcare, Piscataway, NJ) along with the continued inclusion of the test substances.
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