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The volume of the contrast medium we employed in this study was satisfactory for diagnostic purposes ex-vivo.
In our experiments the staining medium we employed contained 3.0 mM EDTA, enough to neutralize 2.7 mM of divalent cations in the staining medium (with a small surplus).
In attempting to obtain secretion of L1 protein at high levels in the culture medium, we employed a codon-optimized α-MF along with the PGK1-based vectors.
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We employed conditioned medium (CM) as a source for factors produced by the human prostate carcinoma cells, PC3 and LNCaP.
Here, we employed a medium sequencing depth (5 20x/site per individual, on average) approach called genotyping by genome reducing and sequencing (GGRS) to detect genome-wide selection signatures of two domestic pig breeds (Yorkshire and Landrace) that have been under intensive selection for traits of muscle development, growth and behavior.
We employed both medium-transfer and cell co-culture approaches to study the RIBE in two types of hSC, that is, hESC and hMSC.
We employed our SD medium and a temperature of 37°C in all experiments.
An alternative explanation is that we employed a different growth medium in our microtiter plate assay and, unlike the earlier study [8], coated the substrate with human plasma.
Such differences, however, would largely result from the different medium being employed.
Usually a water medium is employed in which transmitter, sample, and receiver are immersed.
A trickling biofilter with synthetic fibrous packing medium was employed.
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