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By plating the supernatants obtained on DYGS medium we confirmed that they were free of Azospirillum cells.
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None of the major fermentation products (Table 2) were defined components of the growth medium, and we confirmed that none were introduced to the medium by addition of yeast extract.
This was most likely due to the presence of LPS in the conditioned medium, which we confirmed by adding LPS directly to the endothelial cells and observing similar decline in barrier function (cf. Fig. 6g,h).
Annexin V-positive cells were increased in IL-2 compared with IL-2 containing medium (IL-2. We confirmed our previous data that IL-2 supplementation (rescue) could prevent cells undergoing IL-2-mediated apoptosis.
Furthermore, we confirmed that medium could be perfused into the flow channels quickly (within 10 min) after immobilization of the cells in the assembly.
Several transgenic plant lines were obtained from both plant species by growth on selective medium and confirmed by PCR.
Good consistence between FW H/V with SW H/V of the corresponding cap-layered medium was confirmed.
Secondary thickening of the wall of cells cultured in the induction medium was confirmed by microscopic examination and correlated with an increase in the lignin content and changes in the polysaccharide composition of the cell wall.
However, the copper recovery of bacteria-free cultural supernatant and ferric sulfate solution was similar and much higher than that of pure water and pure medium which confirmed that the copper in PCBs was dissolved by the oxidation of Fe3+.
The induction of elpS in poor medium was confirmed by a β-galactosidase assay performed on a strain carrying the lacZ reporter gene from E. coli K12 under the control of the elpS promoter (Fig. 3E).
The regeneration of plantlets via indirect shoot organogenesis on A10 medium was confirmed using histological techniques.
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