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To test whether PtSIT2-GFP protein expression levels depend on the availability of silicic acid in the medium, we analyzed the reporter fluorescence intensity by flow-cytometry.
To obtain information regarding the rheological properties of the medium
Following incubation of EL- and LacZ-Ad infected cells with HDL (in serum-free medium), we analyzed the phospholipid profile of the incubation media by MS. The analysis revealed a slight but not significant EL-mediated decrease in total PC content (Fig. 1A) as well as in the majority of distinct PC species (Supplementary Fig. 2A), when compared with LacZ-adenovirus infected control cell media.
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In order to rule out the possibility that increased percentages of putative OPCs might results from different proliferation behavior of different cell populations depending on the medium used, we analyzed the absolute numbers of cells and the number of cells positive for each specific antigen used to identify putative OPC, obtaining results consistent with our original interpretation.
To determine, whether the virus-treated cells released infective virus particles into the medium during culture maintenance we analyzed the infective capacity of the culture media removed after 1, 5, 24, 30 or 48 hour long incubation periods.
To validate that the observed "cell density effect" is not due to changes in culture medium during virus production, we analyzed the metabolite profiles of these cultures.
While we analyzed the medium from SHP2-depleted cells, we observed significantly reduced MMP-2.
We analyzed a medium complex metagenome sample of such a microbial community from cow rumen (Hess et al., 2011) with taxator-tk, CARMA3, MEGAN4/5 and PhyloPythiaS (the general model with the 100 most abundant species among sequenced prokaryotes).
In this study, we analyzed the medium-term clinical and RSA results of 39 consecutive CUT femoral neck prostheses implanted for symptomatic osteoarthritis in young patients with heterogeneous and even rare underlying pathologies.
We analyzed complications and medium-term results in patients with simultaneous bilateral total ankle replacement.
These effects were also found when we analyzed MMP release in medium, but were less important (Fig. 5b).
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