Sentence examples for medium we added from inspiring English sources

Moreover, while Liebau et al. [4] added EGF to the culture medium, we added FGF-2, which is not interchangeable with EGF, to the medium to promote cell proliferation.

To the traditional induction medium, we added renal specific growth medium for further induction.

To reduce the diffusion of soluble enzymes in the medium, we added an aliquot of 6% agar.

Because the reporters used here are secreted into the medium, we added compound within 1 h of delivering cells in fresh medium to the microtiter plates (see protocol Supplementary Table 1).

To another 50  μl of Opti-MEM serum-free medium, we added 2  μl of Lipofectamine 2000 (Invitrogen) and allowed it to incubate for 5 min at room temperature.

To this medium, we then added the Notch inhibitor DAPT (Huch et al., 2013b), FGF19 (Wu et al., 2011), and dexamethasone (Rashid et al., 2010).

To deplete the medium of survivin, we added anti-Flag beads (20  μl ml−1) to the medium and rotated overnight at 4°C.

The inoculum consisted of a preculture of Y. lipolytica in YEPD medium, of which we added a volume corresponding to 10% of the total fermentation volume.

After that, we added medium containing Y-27632 ((R --trans-N- 4-Pyridyl -4- 1-aminoethyl)- cyclohexanecaR --trans-N- 4-Pyridyl -4- 1-aminoethyle Sciences, Inc). in 10, 50, 100 μM, R --trans-N- 4-Pyridyl -4- 1-aminoethyl

At the end of 4½ days, we added medium containing fetal bovine serum (FBS) plus SF plus IL-3 plus IL-6 plus erythropoietin (Epo) as a further stimulus to promote the formation of readily detectable differentiating clones from persisting viable cells.

One day post-infection we aspirated the inoculum and after 5 PBS washes we added fresh medium with 2% DMSO but without PEG.