Sentence examples for medium washing from inspiring English sources

After 24 h of cell proliferation, the cells were exposed to the nanoparticles by aspirating the medium, washing the cells with PBS and adding the nanoparticle-containing medium.

After 5 7 days, cells were passaged by aspiration of medium, washing with PBS, and incubation with PBS for 5 min at 37°C.

Briefly, tumour tissue sample, after a medium washing step, was first finely cut with scalpel blade and crushed.

Cells were cultured undisturbed for a further 7 days before removal of medium, washing with PBS and fixation with 70% EtOH.

Cells were collected every 2 hours by removing medium, washing in PBS, treating with 100 µl 0.25% trypsin-EDTA, and collecting in 1 ml medium.

Ten days after seeding, cells were fixed by removing medium, washing with PBS and adding 4% paraformaldehyde in PBS at room temperature for 30 min.

Serves 4. leeks 3, medium, washed, cut in half and thinly sliced garlic 1 clove anchovy fillets 4 Sea salt and black pepper fresh ricotta 150g parsley a handful, chopped orecchiette 320g butter 2 tbsp extra-virgin olive oil Thinly slice the garlic and fry in olive oil over a medium to high heat.

Cells were first grown overnight in PIB medium, washed twice with one volume of 1x M9, and resuspended in an equal volume of the same medium.

Each strain was incubated overnight at 30°C in YPD medium, washed, serially diluted (1 to 104 dilutions) in dH2O, and spotted (4 µl) onto solid YPD medium.

Cortical neurons were loaded with the Fura-2 Ca2+ indicator in 2 mM CaCl2-containing medium, washed, and transferred to a 0.5 mM EGTA medium to initiate measurements.

Cells expressing various levels of Metnase were treated with VP-16 for 8 hr or adriamycin for 4 hr in growth medium, washed twice, fresh growth medium replaced, and incubated at 37°C with 5% CO2 for 10 14 days.