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Conditioned medium was transferred to naïve cells, and viral p24 protein in the culture supernatant was measured by ELISA.
The mycelium stored on PDA medium was transferred to new PDA medium in 9-cm diameter Petri dish and incubated at 30°C for 5 days.
After incubation for 2 h at 37°C, 100 μl medium was transferred from each well to a new black 96-well plate.
After 70 h, each medium was transferred to a 500 mL screw-cap flask and centrifuged at 3000 rpm for 20 minutes.
This medium was transferred into sterilized Petri dishes in a laminar air flow chamber (Microfilt Laminar Flow Ultra Clean Air Unit, Mumbai, India).
A 15-ml volume of each sample from each of the medium was transferred to a 50-ml tube and incubated at 25 °C with shaking at 100 rpm for 48 h.
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The flasks containing the cells in fresh medium were transferred aseptically to the bioreactor to start new batch fermentation.
(b) Radicle emergence was recorded and data are means ± SD (n = 30 35 plants) (c) One-week-old seedlings grown on standard medium were transferred to culture plates with concentrations of mannitol (0 300 mM) for 7 days.
When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis.
Seven-day-old seedlings grown on MS medium were transferred to the medium with various NO3 −, Ca2+ and K+ concentrations, and grown for 14 days in a growth chamber at 24 °C under 8 h of darkness and 16 h of light at 150 μmol photons m−2 s−1.
Yeast transformants (Leu+, Trp+, His+) grown on minimal medium were transferred onto filter papers.
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