Suggestions(1)
Exact(3)
The cell medium was thereafter changed at least 5 times/week.
Medium was thereafter changed to RPMI (Mediatech) supplemented with 0.5% pen/strep, 0.5% glutamate and 100 ng/ml activin A (R&D) for one day.
P31 and H1299 cells were cultured to about 80% confluence and the medium was thereafter changed to fresh medium containing 0 or 5 mg l 1 cisplatin and/or 0.1 μg l 1 VT-1, and incubation continued for 72 h.
Similar(57)
SLPI concentrations in the cell medium were thereafter detected with ELISA.
The 5-Aza-CdR was removed from the culture medium at 24 h, and regular medium was used thereafter.
Medium was not changed thereafter.
After 5 days the culture medium was exchanged and thereafter every 3-4 days until the culture reached 80-90% confluence.
More precise methods for load prediction onto piping, small and medium sized equipment are thereafter proposed and evaluated.
Thereafter, medium was changed in the basolateral compartment every 48 h until day 21 of ALI culture.
Discard of non-adherent cells and first medium change were performed after 2 days: thereafter medium was changed three times a week.
Transduced cells were incubated for 24 hrs and thereafter medium was replaced with fresh IMDM and cells were incubated for another 24 hrs.
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