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The medium was subsequently refreshed every other day.
M9-based culture medium was subsequently optimized for the enzyme production by orthogonal experimental design in shake flasks.
Medium was subsequently replaced with WEM supplemented with Cell Maintenance Supplement Pack (Invitrogen) and 50% of the media volume was replaced every 48 h.
The culture medium was subsequently discarded and replaced with 150 μL of DMSO.
The filtered TSB medium was subsequently incubated at 30°C for 14 days.
Fresh medium was subsequently added, and the cells were shifted to 37 °C to allow synchronous infection.
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Virus-containing medium is subsequently harvested at ~24 and 48 h thereafter.
The hydrophilic analytes in the then-organic medium were subsequently extracted by the HILIC trap column.
Aliquots of medium were subsequently removed and analyzed spectrophotometrically at a wavelength of 560 nm.
ECs incubated with the respective medium were subsequently obtained after 24 h and 48 h under normal gravity incubation and numbers were calculated.
TMB medium, originally described by Eschenbach et al [ 15] and optimized by Rabe & Hillier [ 7], demonstrates the ability of a LB to produce H2O2 when colonies grown anaerobically on the medium are subsequently exposed to air.
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