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The medium was stored at 4 °C without delay.
The medium was replaced on day 1 and 3; and the retrieved medium was stored at −20 °C until progesterone assay.
The supernatant was removed and the wells were washed with DMEM-F12 without inactivated FBS; this conditioned medium was stored to be used later.
This concentrated medium was stored at −20°C in aliquots.
The transport medium was stored at 4°C until use.
The conditioned medium was stored at -20°C until analysis.
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Aliquots of medium were stored at –80°C until analysis.
Whole stool and inoculated transport medium were stored at 2 8°C for up to 3 days before transport to LNSP.
The conditioned medium was collected after 72 h of incubation and centrifuged at 2000 g for 10 min, and aliquots of cultured medium were stored at −80 °C before analysis.
Our experience has been that the level of nuclease activity present in tissue culture FBS is variable and disappears over days to weeks when prepared medium is stored at 4 °C.
The therapeutics erlotinib, trastuzumab and pertuzumab, abbreviated by first letters, that were permanently active in the simulated perturbation conditions besides the stimulus S, standing for the full growth medium, are stored in the column Simulation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com