For the incubation at t = 308 days, the medium flow was stopped and nitrite was added to a final concentration of 1.3 mM in the bioreactor.
Cells were fed every 48 hr with control, or RA- containing fresh medium and the treatment was stopped 2, 4, 6 or 12 days after the first addition of RA.
Trypsinization was stopped with medium containing 10% FBS, and the cells were washed once in serum-free medium and resuspended in PBS (phosphate buffered saline) as vehicle.
Trypsinization was stopped with medium containing 10% FBS, and the cells were washed once in serum-free medium and resuspended in PBS.
To enable visualization of cells within the biofilm the medium supply to the flowcell was stopped and each channel was stained for 30 min with 1.0 ml DNA staining solution (5 µM SYTO-9 in LB-10; Invitrogen, U.K ., followed by a period of 10 min with medium flow to wash out excess stain.
Trypsinization was stopped with medium containing 10% FBS.
For the injection of 6606PDA cells, subconfluent cultures of cells were trypsinized and the trypsinization was stopped by medium.
Vettel would almost certainly have been in the top three overall if he had not had his first lap on the mediums wrecked when the session was stopped because Manor's Roberto Merhi was beached in a gravel trap at Turn 11.
The medium was removed and the reaction was stopped by adding 0.1 M HCl.
Treatment was stopped with differentiation medium and the cells were centrifuged (210 g, 5 min).
Following digestion, the reaction was stopped with expansion medium and cell suspension was centrifuged (400 × g, 10 min).
