Sentence examples for medium was started from inspiring English sources
Medium was started by Twitter co-founder Ev Williams.
Selection with 250 μg/ml hygromycin-containing medium was started 24 hours after plating on the 9-cm dishes.
The £28.6m spent in 2008 represents a 99% annual rise, which indicates that the medium is starting to gather pace, albeit from a low base.
They hark back to a time when flatness was abstract painting's primary goal, and the physical facts of the medium were starting to be endlessly parsed — beginning with shaped canvases — in a process that continues today.
The medium is starting to catch up with the message although I don't even pretend to know what the message is.
Cultures were inoculated to an initial OD600 of approximately 0.5 and maintained as batch cultures for 6 to 9 h, after which continuous medium feed was started while the cells were still growing exponentially.
The 100-mL holding volume of ODM with 60 g/L xylose and 20 g/L ethanol was inoculated to A620 0.5, and the population was allowed to grow batch-wise to stationary phase; then the feed medium flow was started.
To prevent these, approximately 5% of the total cell concentration in the bioreactor was added to the culture as cells in mid to late exponential phase at the time when continuous medium feed was started [ 73].
On day 1 culture in medium containing ddC was started, followed by dsRNA transfection on day 3 using LIII dsRNA or control Sc dsRNA.
For differentiation, 8 × 10 LUHMES were seeded into a T175 flask in proliferation medium and differentiation was started after 24 h on day 0 (d0), by changing to advanced DMEM-F12, 1× 'N2 supplement', 2 mM l-glutamine, 1 mM dibutyryl 3′,5′-cyclic adenosine monophosphate (cAMP), 1 μg/ml tetracycline and 2 ng/ml GDNF.
When cells were ~50% confluent (usually 2 days after plating for MCF-7 cells and 3 days after plating for MDA MB-231 cells), the medium was changed and treatment with compounds was started in fresh medium.
