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The medium was slowly injected at a constant rate of 0.5 μL/min.
Then 20 30 mL of serum-free DMEM/F-12 culture medium was slowly added to the tube and the cells were carefully mixed.
To remove SPION weakly bound to the cell surface or the filter, 3 × 10 mL of 10 mM ethylenediaminetetraacetic acid in BG-11 medium was slowly passed through the filter.
While gently shaking, 1 μg of plasmid in 100 μL of RPMI medium was slowly added into 2 μL of TurboFect™ and incubated at room temperature for 15-20 minutes.
siRNA oligonucleotides were added to a final concentration of 25 nM and the mixture was incubated at room temperature for 5 20 min. The transformation medium was slowly added to the wells and incubated for 72 h in a CO2 incubator under standard growth conditions.
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Gasification medium is slowly flow through a fixed bed of solid particles.
However, if the medium is slowly varying, one might hope that there exist approximate solutions under certain conditions corresponding to the characteristic modes that are at least less strongly coupled to each other than the Cartesian field components.
After centrifugation the mononuclear cells formed a distinct band at the medium interface and was slowly removed using pasteur pipette without removing the upper layer.
We confirmed that the extent of arterial injection covered the tumour by a pigment, angiography, and MRI from 2001, in which an extremely low dose of contrast medium for MRI was slowly infused via a catheter for arterial injection.
In brief, one mL of the medium containing 80% density was placed in a test tube, and then 1 mL from the same medium containing 55% density was slowly added on the side of the tube to prevent mixing between the two layers of the media and to have a clear separation between them.
Then 10 mL of contrast medium was deposited slowly into the capsule-sheath space with frequent aspirations over 1 2 min.
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