Sentence examples for medium was seeded from inspiring English sources

For those cells treated with miRNA after transfection for 48 h, cells were seeded at 1 × 10 cells/ml with serum-free medium, 100 μl cell suspension with serum-free medium was seeded to the upper chamber, cells were stained with crystal violet staining solution (Beyotime, Nantong, China) then counted and photographed under 40× magnification (five views per well).

Transfected cells grown in complete medium were seeded into six-well plates.

The iPS-MSCs in fresh culture medium were seeded 1×105 cells per well in triplicate in six-well plates.

Three milliliter medium were seeded with the culture and grown for 5 h with 1 μg/ml Doxy at 25 °C in a shaker (255 rpm).

For evaluation of nanoparticle transfer across placental cell barriers, 1 * 105 cells cm−2 in 0.5 ml complete growth medium were seeded on apical transwell chambers and incubated.

About 4 × 107 osteoblast precursor cells in 200 μl cell-culture medium were seeded into each of six scaffolds and incubated in vitro for 2 days.

In order to determine the invasion of MCF-7 cells in co-culture with fibroblasts, 2 × 104 MCF-7 cells in 200 μl serum-free medium were seeded into the upper well of the Boyden chambers coated with ECM.

Briefly, 1 × 104 fibroblasts (CAFs, AHFs, NFs, or engineered fibroblasts) in 200 μl serum-free medium were seeded into the Boyden chambers of 8 mm pore size (Corning) coated with ECM (1 7.5) (Sigma).

First, the basal chamber of the transwell inserts was filled up with 1.5 ml of cell culture medium on day 0. Next, 1 * 105 cells cm−2 in 0.5 ml culture medium were seeded onto the 3 μm porous polyester membrane of the apical chamber.

5 × 103 3T3-L1 preadipocytes suspended in 100 μL of growth medium were seeded in 96-well plates and let cells grow to confluence.

3T3-L1 preadinocytes in 100 μL growth medium were seeded in 96-well plates at a density of 5000 cells per well.