Wild-type (WT) E. coli transformed with an empty vector remained sensitive to the presence of uracil or 5FU, whereas the growth of this same dut-1 mutant on uracil or 5FU containing medium was restored to a similar level as the WT E. coli upon transformation with the AtDUT1 cDNA (Figure 1A).
The auxin response (hypocotyl elongation) was compromised in gai-1 and on the PAC medium (high DELLA condition), but the reduced auxin sensitivity on the PAC medium was restored in della (low DELLA condition) indicating that DELLA inhibits the auxin promotion of hypocotyl elongation.
Among the messages portrayed through this medium were, "restore integrity," "raised right," and "Hillary for prison 2016".
After each gefitinib exposure, remaining cells were cultured in gefitinib-free growth medium until stable growth was restored.
With further culture (13 days) in serum-containing medium, the GAG content was restored, and collagen density and tensile properties increased [ 34].
After feeding was interrupted, the cells started to consume the excess glycerol (and salts) accumulated in the medium, and cell growth was restored at the end of induction phase, leading to a final biomass concentration of 120 gDCW L-1.
Indeed, as depicted in Figure 5J, the proliferation of HUVECs was restored in conditioned medium from orlistat-treated SK-MEL-25 cells containing anti-VEGFA165b antibodies, strongly suggesting an anti-angiogenic role for the orlistat-induced secretion of VEGF165b by tumour cells.
When K+ was restored to the medium by the addition of 10 mM KCl, [Na+]i recovered to a level not significantly different from the initial resting [Na+]i (recovery to 9.5 ± 2.7 mM; n = 15; p = 0.15), and pHi increased, albeit not quite to the initial resting value.
The complementation assay demonstrated that in all three overexpression lines the capacity to grow on a medium supplemented with 0.5 mM and 1 mM urea was restored.
Similarly, shASNS-infected cells exhibited only minimal OP-puromycin staining, while synthesis of new polypeptides was restored in shASNS-infected sarcoma cells grown in medium supplemented with asparagine.
When the LG medium without ethanol supplementation was switched back, the process oscillations were attenuated, and the steady state previously observed was restored.
