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The culture medium was replenished twice per week.
After 24 h of incubation, the medium was replenished.
Cells were seeded in complete cell culture medium and growth at 37°C in a humidified incubator (HERAcell 150; Heraeus, Hanau, Denmark) with atmosphere containing 5% CO2, and culture medium was replenished every 2 days with a fresh medium.
Medium was replenished every other day.
Half of the culture medium was replenished every 3 days.
50% of the culture medium was replenished after 5 d.
Cell culture medium was replenished every 2 3 days.
The culture medium was replenished every second days.
Medium was replenished with new drug of 5-AZA-dC or BaP every 48 hr.
The growth medium was replenished every 3 4 days until confluent.
The culture medium was replenished twice a week by replacing half the volume.
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