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Medium was recovered and saline buffer was added to harvest the cells.
After incubation medium was recovered and precipitated with an equal volume of 10% tricholoroacetic acid.
At 2 day increments, conditioned medium was recovered for ApoE ELISA.
After 24 hours, the medium was recovered and stored at -80°C for further analysis.
The culture medium was recovered, lyophilized, and resuspended in SDS-PAGE sample buffer.
The supernatant of YPD medium was recovered by centrifugation and also used as negative control.
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Optical tomography is mathematically treated as a non-linear inverse problem where the optical properties of the probed medium are recovered through the minimization of the errors between the experimental measurements and their predictions with a numerical model at the locations of the detectors.
Viruses growth kinetics were assessed infecting BEK, BEK-IE1 and BEK-boBST2 cells with 0.1 m.o.i. of virus, cells were monitored periodically for 72 hs at the microscope to verify the CPE appearance and small aliquots of medium were recovered at 24, 48 and 72 hs and titrated in BEK cells.
Conditioned medium (CM) was recovered from cells cultured in medium without FBS for at least 24 h.
The culture medium of CNE cells was recovered for ELISA assays using VEGF ELISA from R & D systems (Minneapolis, MN) and a GENios ELISA plate reader (TECAN, Austria).
The medium of each culture was recovered every 24 hr and stored at −80°C until a testosterone radioimmunoassay could be performed (Testosterone Direct RIA kit; Beckman Coulter, Villepinte, France).
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