Sentence examples for medium was reconstituted from inspiring English sources

The culture medium was reconstituted just before use by pouring sterile 5% sodium bicarbonate in ratio of 1 24 and the culture was further supplemented with 10% Bovineserum.

The skim milk medium was reconstituted (10% w/v) from milk powder (Katnarat, Armenia) and then sterilized at 110 °C for 15 min.

Instant Drosophila medium (Carolina Biological Supply Company) was reconstituted in each vial with a solution of 1% yeast in water, supplemented with either increasing concentrations of drug diluted in DMSO (Sigma D2650), or DMSO alone.

The protein fraction was reconstituted in fresh medium to the same volume as the initial sample.

After vortexing and centrifugation (1000 rpm, 5 min) the pellet containing bone plugs and released cells was reconstituted in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS), antibiotics (50 I.U. penicillin/ml and 50 μg streptomycin/ml) and 50 μg/ml ascorbate (complete medium).

Lyophilised rP11-4 (10 mg/ml) was reconstituted in either cell culture medium (DMEM) or 140 m m NaCl in water (pH 7.4).

Collagenase was reconstituted in Dulbecco's Modified Essential Medium (DMEM; high glucose, with L-glutamine, 110 mg/l sodium pyruvate, and pyridoxine hydrochloride; supplemented with 50 U/ml penicillin and 50 mg/ml streptomycin; GIBCO Invitrogen).

Dihydroethidium (Dhe) dye was reconstituted in 1 ml Schneider's medium to give a final concentration of 30 µM.

Fibrinogen was reconstituted in 37 °C serum-free culture medium and added directly to the wells in a concentration of 5 mg/ml as previously described [ 11, 25, 30].

For calcium measurements, aequorin was reconstituted by the overnight incubation of the cell suspension in Gamborg medium containing 2.5 µM native coelenterazine.

The vaccination strain used was M. bovis BCG Danish strain 1331 (SSI, Copenhagen, Denmark) which was reconstituted from freeze-dried stocks stored at 4°C in Sauton's medium supplied as per instructions.